engleski

Articial urine – possibility or a good try

Background: Based to our knowledge, external quality control assessment for urinalysis in most schemes is organized through sending pictures of urine sediment into laboratories. Although useful in testing of element recognition, this approach doesn’t cover the whole process of urine sediment preparation. The aim of our study was to prepare artificial, low infective sample in order to test whole process of urine sediment analysis. Materials and methods: We suspended small amount of human blood, epithelial cells from buccal mucosa and baker's yeast in saline fluid. This mixture was distributed into 13 samples and given for sediment analysis to a 13 professionals into 4 laboratories on the same day. Results: As we expected, most of professionals managed to recognize all elements added into saline. Overall agreement for erythrocytes was 13/13, for epithelial cells 12/13, for leukocytes 10/13 and for yeasts 9/13. Relatively low agreement on yeast recognition might be due to growth of cells in samples that were examined last and also because of similarity of these elements to erythrocytes. Raters that didn’t find any leukocytes are from laboratory that reported smaller amount of urine used for analysis. Some of raters reported artefacts and salts that were not present in the samples. Also, they were confused with morphology of yeasts and complained about morphology of erythrocytes. Conclusion: This approach has potential to develop into quality control of the total process of urine sediment analysis. However, because the transport conditions could influence composition of samples it could be applied locally.

internal quality assessment ; urinanalysis

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