engleski

Electron microscopy in diagnostic practice

The aim of the present review is to highlight the importance, placement and availability of the electron microscopy (EM) analyses in routine diagnostic work with biopsy samples. Electron microscopy is a relatively new method dating from the 1970es, when it was primarily used in the diagnosis of tumors of unknown primary sites. Today it has a much wider application, such as in kidney, congenital, metabolic and accumulation diseases, neuromuscular disorders and numerous other conditions. Prerequisite for adequate EM analysis is good knowledge of ultrastructural cellular formation of which the article gives a brief review. In addition, the pre-analytical phase for EM analysis, which includes proper sampling, adequate processing of tissue and acceptable the sections, necessary for satisfactory analysis is also described in the paper. As the method is the method is relatively complex and financially demanding the use of EM should be. Finally, the article describes some of the most important indications for the use EM in routine work, documented with images, such as in glomerular and muscular diseases and tumor pathology, where light microscopy and immunohistochemical analysis, are very often not sufficient for the final diagnosis.

Diagnostics; Electron microscopy

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