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NON-THERMAL EFFECT OF 1800 MHz RADIATION ON HUMAN NEUROBLASTOMA CELL (CROSBI ID 637402)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija

Marjanovic, Ana Marija ; Pavicic, Ivan ; Trosic, Ivancica NON-THERMAL EFFECT OF 1800 MHz RADIATION ON HUMAN NEUROBLASTOMA CELL // Book of Abstracts of the Congress of the Croatian Society of Biochemistry and Molecular Biology / Katalinić, Maja ; Kovarik, Zrinka (ur.). Zagreb: Hrvatsko Društvo za Biotehnologiju, 2016. str. 90-90

Podaci o odgovornosti

Marjanovic, Ana Marija ; Pavicic, Ivan ; Trosic, Ivancica

engleski

NON-THERMAL EFFECT OF 1800 MHz RADIATION ON HUMAN NEUROBLASTOMA CELL

Aim of this study was to assess possible connection between short-term non-thermal RF exposure and development of oxidative stress. Human neuroblastoma cells (SH-SY5Y) were exposed to GSM modulated 1800 MHz radiation for 10, 30 and 60 minutes inside of Gigahertz Transversal Electromagnetic Mode Cell (GTEM). Electric field strength inside GTEM cell was 30 V/m and specific absorption rate (SAR) was calculated to be 1.6 W/kg. Cellular viability was measured by 3-(4, 5-dimethylthiazole-2-yl)-2, 5-diphenyltetrazolium bromide assay (MTT) and level of reactive oxygen species (ROS) was determined by fluorescent probe 2′, 7′-dichlorofluorescin diacetate (DCFH-DA). Lipid oxidative damage in the cell samples was assessed by measuring concentrations of malondialdehyde (MDA). Viability of exposed cells remained within normal physiological values regardless of exposure duration. Compared to control, level of ROS in irradiated cell samples was found to be significantly higher for every exposure time. Increased concentration of MDA (p<0.05) was observed after 60 minutes radiation exposure. In conclusion, results of our study suggest that short-term exposure to 1800 MHz RF radiation increases ROS production in neuroblastoma cells causing oxidative damage to cell membranes

RF radiation; SH-SY5Y cells; GTEM cell; ROS

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o prilogu

90-90.

2016.

objavljeno

Podaci o matičnoj publikaciji

Book of Abstracts of the Congress of the Croatian Society of Biochemistry and Molecular Biology

Katalinić, Maja ; Kovarik, Zrinka

Zagreb: Hrvatsko Društvo za Biotehnologiju

Podaci o skupu

Congress of the Croatian Society of Biochemistry and Molecular Biology

poster

01.06.2016-04.06.2016

Split, Hrvatska

Povezanost rada

Temeljne medicinske znanosti, Biologija