Reg genes expression in regenerating skeletal muscle (CROSBI ID 637709)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Starčević-Klasan, Gordana ; Ivanac, Danijel ; Jurišić-Eržen, Dubravka ; Jerković, Romana
engleski
Reg genes expression in regenerating skeletal muscle
The Reg genes have been well documented to play an important role in various tissue regeneration processes, cell proliferation, differentiation and carcinogenesis. However, the regulation of Reg gene expression in rat skeletal muscles regeneration process is poorly understood. Therefore, the aim of this investigation was to reveal the transcriptional activation of Reg genes in the skeletal muscles in response to injury. In 2-month-old Wistar rats three experimental models of muscle and nerve injury were used. The regeneration process was induced in both types of muscles, slow (m. soleus, SOL) and fast (m. extensor digitorum longus muscle, EDL) by injection of 1 ml of local anesthetic, by denervation while in third experimental model muscle denervation and injection of local anesthetic were combined. The RT-PCR analysis was performed for detection of Reg1, Reg3G and Reg4 mRNA in regenerating muscles. RT-PCR analysis showed that Reg1 gene is expressed in EDL and SOL with peak expression 12 hours after injury and signal intensity was constant till 7th day in all three experimental models. Reg3G can be detected over a longer period in comparison with Reg1 and signal intensity was lower. There was no detectable Reg4 mRNA expression in either SOL or EDL muscle. Our investigation revealed presence of Reg1 and Reg3G genes in regenerating skeletal muscle and its expression is up regulated with regeneration process. Reg1 and Reg3G genes expression was induced in both skeletal muscles at a considerably early time point after muscle injury. Our results imply that Reg genes have a significant role at the early stage of skeletal muscle regeneration.
Reg gene; skeletal muscle; regeneration
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Podaci o prilogu
129-129.
2014.
objavljeno
Podaci o matičnoj publikaciji
Revista Argentina de Anatomia Clinica
Biasutto, Susana N
Cordoba: Asociación Argentina de Anatomía Clínica
1852-8023
Podaci o skupu
The 6th International Symposium of Clinical and Applied Anatomy
predavanje
26.06.2014-29.06.2014
Malinska, Hrvatska