engleski

The Effect of p73 Isoforms on DNA Methylation in Cancer Cells

p73 exists in multiple isoforms which could be divided into two groups: one containing transactivation domain (TA) and another amino- terminally truncated (ΔN) isoforms. While TAp73 isoforms show tumor-suppressive functions similar to those of wild-type p53, ΔNp73 isoforms inhibit wild-type p53 as well as TAp63 and TAp73, and are considered as potential oncogenes. ΔNp73 isoforms are overexpressed in many tumors correlating with enhanced chemoresistance and poorer disease outcome. DNA methylation is a form of epigenetic regulation, which adds methyl groups to cytosine residues, regulating gene activity. Hypermethylation of CpG islands in promoter regions inhibits transcription of genes silencing its expression. Using inducible tet-on system, we examined the impact of increased expression of TAp73α and ΔNp73α on global DNA methylation using Illumina Human Methylation 450 BeadChip. Statistically significant change was not found upon induced expression of any p73 isoform.

TP73 ; p73 isoforms ; TAp73α ; ΔNp73α ; methylation

nije evidentirano