Impact of L-arginine (a nitric oxide synthesis precursor), and N--nitro-L-arginine-methyl- ester (L-NAME) (a non-selective nitric oxide synthase inhibitor) Chronic, Subchronic, and Acute Toxicity-Induced Lesions on Ascites– Pulmonary Hypertension Syndrome Development in Broiler Chickens (CROSBI ID 638469)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Tišljar, Marina ; Grabarević, Željko ; Artuković, Branka ; Džaja, Petar ; Foršek, Jadranka ; Barić Rafaj, Renata
engleski
Impact of L-arginine (a nitric oxide synthesis precursor), and N--nitro-L-arginine-methyl- ester (L-NAME) (a non-selective nitric oxide synthase inhibitor) Chronic, Subchronic, and Acute Toxicity-Induced Lesions on Ascites– Pulmonary Hypertension Syndrome Development in Broiler Chickens
Experiment A. Chronic toxicity. 140 one day-old male broiler chicks divided in four groups. Every other day: intraperitoneal (ip) L-NAME (LN, 10 mg/kg BW) ; L-arginine (LA, 100 mg/kg BW), L-arginine and a L-NAME combination (100 and 10 mg/kg BW, respectively), and physiological saline (0.90% w/v ; 0.5 mL/kg BW). Seven birds were euthanized weekly during their first five weeks of life. Experiment B. Subchronic toxicity. Equal doses of the same substances: ip every eight hours into 38 day-old birds over 36 hours. Experiment C. Acute toxicity. Thirty day-old birds: one ip dose of various concentrations of L-NAME (50, 100 and 150 mg/kg BW) ; L-arginine (100 mg/kg BW) and saline (0.5 ml/kg BW). All the birds were euthanized after six hours. The end of experiment A: the gross finding of ascites-pulmonary hypertension syndrome (PHS) confirmed vasoconstrictory effect of L-NAME in five birds (LN). Toward the end of experiment A ; in experiment C: histopathological findings (myocardial/pulmonary oedema /LN/ ; congestion/ haemorrhages /LA/) the most prominent (higher LN-doses /100 and 150 mg/kg BW/). Irreversible myocardiolysis and hepatocellulolysis: confirmed in all three experiments (LN). Focal myocardial degeneration solely: in the L- NAME/L-arginine simultaneously treated group. Haematological and blood chemistry values ; stress index value ; and relative organ weights agreed with well-known literature data on PHS. Experiments A and C. The severity of LN- provoked hypoxic changes and plentiful haemorrhages (LA) - dose and time dependent. Mild changes in L-NAME/L-arginine group confirmed a protective role of LA.
nitric oxide; L-arginine; L-NAME; broiler chickens; ascites - pulmonary hypertension syndrome (PHS)
http://dx.doi.org/10.4172/2157-7579.C1.016 - Journal of Veterinary Science and Technology, 7 (5) (Suppl), August 2016, Open Access
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Podaci o prilogu
84-84.
2016.
objavljeno
Podaci o matičnoj publikaciji
Proceedings of 3rd International Veterinary Congress, August 18-20, 2016, conferenceseries.com 625th Conference
London : Delhi: Conference Series LLC, USA ; Conference Series, UK
Podaci o skupu
3 rd International Veterinary Congress
ostalo
18.08.2016-20.08.2016
London, Ujedinjeno Kraljevstvo