engleski

Recovery of phenolic acids and enzymes production from corn silage biologically treated by Trametes versicolor

Corn silage is used as high-energy forage for dairy cows, and more recently for biogas production in a process of anaerobic co- digestion with cow manure. In this work fresh corn silage after the harvest was used as a substrate in solid-state fermentations with T. versicolor with the aim of phenolic acids recovery and enzymes (laccase and manganese- peroxidase) production. During 20 days of fermentation, 10.4, 3.4, 3.0 and 1.8-fold increments in extraction yield of syringic acid, vanillic acid, p hydroxybenzoic acid and caffeic acid, respectively were reached when compared to biologically untreated corn silage. Maximal laccase activity was gained on 4th day of fermentation (V.A. = 180.2 U/dm3) and manganese-peroxidase activity was obtained after 3rd day of fermentation (V.A. = 30.1 U/dm3). The addition of copper(II) sulfate as inducer during solid state fermentation, resulted in 8.5 and 7-fold enhancement of laccase and manganese-peroxidase activities, respectively. Furthermore, the influence of pH and temperature on enzymes activities were investigated. Maximal activity of laccase was obtained at T = 50 °C and pH = 3.0, while manganese-peroxidase is active at temperature range T = 45 - 70 °C with the maximal activity at pH = 4.5.

corn silage ; solid-state fermentation ; Trametes versicolor ; phenolic acids ; laccase ; manganese-peroxidase

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