engleski

Development of biodegradable injectable system based on chitosan and calcium phosphate

Injectable gels have gain great importance due to their potential to minimize invasive manner during that surgical therapy. Injectable gels have ability for gelation under physiological conditions and as such are used for cell delivery and nutrients and growth factors diffusion. Injectable hydrogels based on chitosan as a biodegradable polymer matrix and hydroxyapatite as a bioactive inorganic phase are prepared in this study. The gelation of chitosan-hydroxyapatite hydrogels has been initiated by sodium hydrogencarbonate (NaHCO3). The composition of dried injectable hydrogels was determined by X-ray diffraction analysis and Fourier transmission spectroscopy, while morphology of lyophilized gels was investigated by scanning electron microscopy. Reological behaviour of hydrogels was determined by amplitude and frequency sweep experiments. Cytotoxicity of injectables was evaluated using MTT method. The characterization of prepared hydrogels has confirmed formation of hydroxyapatite within chitosan matrix. The reological measurements of chitosan- hydroxyapatite gels have indicated rapid thermogelling of prepared system and confirmed formation of 'strong physical gel' with suitable shear modulus. Biological evaluation confirmed the non-cytotoxicity of the prepared hydrogels. The composition of prepared hydrogels is favourable for bone tissue regeneration, while gelation rate makes those gels applicable as a cell carrier.

chitosan ; hydroxyapatite ; injectable hydrogel ; rheology ; cell proliferation

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