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THE EFFECT OF PROTEOLYTIC PROCESSING ON THE LOCALIZATION AND PHYSIOLOGICAL ACTIVITY OF THE Saccharomyces cerevisiae CELL WALL PROTEIN Scw4p (CROSBI ID 640175)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | domaća recenzija

Teparić, Renata ; Grbavac, Antonija ; Kunštek, Sandra ; Mrša, Vladimir THE EFFECT OF PROTEOLYTIC PROCESSING ON THE LOCALIZATION AND PHYSIOLOGICAL ACTIVITY OF THE Saccharomyces cerevisiae CELL WALL PROTEIN Scw4p // Book of Abstracts of Congress of the Croatian Society of Biochemistry and Molecular Biology "The Interplay of Biomolecules", HDBMB2014 / Katalinić, Maja ; Kovarik, Zrinka ; (ur.). Zagreb: Hrvatsko Društvo za Biotehnologiju, 2014. str. 134-134

Podaci o odgovornosti

Teparić, Renata ; Grbavac, Antonija ; Kunštek, Sandra ; Mrša, Vladimir

engleski

THE EFFECT OF PROTEOLYTIC PROCESSING ON THE LOCALIZATION AND PHYSIOLOGICAL ACTIVITY OF THE Saccharomyces cerevisiae CELL WALL PROTEIN Scw4p

Yeast cell wall contains proteins that are noncovalently (Scw- proteins) or covalently (Ccw-proteins) bound to β-1, 3-glucan, either through GPI-anchors and β-1, 6-glucan, or by alkali labile ester linkages between γ-carboxyl groups of glutamic acid and hydroxyl groups of glucoses (Pir-proteins). It was previously shown that one of the most abundant Scw protein, Scw4p is partly also covalently linked to the cell wall and that non- covalently bound Scw4p underwent the proteolytic processing, while the covalently bound Scw4p was not processed. Such finding indicates that the proteolytic processing might determine the localization of these two forms of the protein. Proteolytic enzymes which might have a role in processing of Scw4p are Kex2p and a family of aspartic proteases called yapsins. To get a better insight in the processing of Scw4p, different mutations were introduced to SCW4 in the region of predicted processing sites. On the other hand mutant strain was constructed missing all yapsin proteases and Kex2p (5yps∆kex2). Native and mutated forms of Scw4p were expressed in kex2 yeast strain, strain with all yapsin genes disrupted (5yps∆) and strain with all proteases disrupted (5yps∆kex2) and Scw4p processing was examined. Furthermore, phenotypes of strains overproducing native and genetically modified Scw4p forms were examined.

Scw4p; Kex2p; yapsins

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Podaci o prilogu

134-134.

2014.

objavljeno

Podaci o matičnoj publikaciji

Book of Abstracts of Congress of the Croatian Society of Biochemistry and Molecular Biology "The Interplay of Biomolecules", HDBMB2014

Katalinić, Maja ; Kovarik, Zrinka ;

Zagreb: Hrvatsko Društvo za Biotehnologiju

978-953-95551-5-1

Podaci o skupu

The Interplay of Biomolecules, HDBMB2014

poster

24.09.2014-27.09.2014

Zagreb, Hrvatska; Zadar, Hrvatska

Povezanost rada

nije evidentirano