Biological activity of isolated compounds from Centaurea ragusina L. (CROSBI ID 640224)
Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Vujčić, Valerija ; Radić Brkanac, Sandra ; Radić Stojković, Marijana ; Žilić, Irena ; Tolić, Sonja ; Krivohlavek, Adela ; Ivanković, Siniša ; Ivanković, Dušica ; Stojković, Ranko ; Hrenović, Jasna ; Ruščić, Mirko ; Grienke, Ulrike ; Rollinger, Judith Maria
engleski
Biological activity of isolated compounds from Centaurea ragusina L.
Antioxidant, antibacterial, and cytotoxic activity of three flavonoids (chrysin, oroxylin A, and hispidulin) and sesquiterpene lactones (hemistepsin A, deacylcynaropicrin and (3aR, 4S, 6aR, 8S, 9aR, 9bR)-[Dodecahydro-8- dihydroxy-3, 6, 9- tris(methylene)-2oxo-2(3H)- azuleno[4, 5-b]furanyl]-3-methyl-butanoate) isolated from ethanolic extract of the Croatian endemic plant species Centaurea ragusina L. were studied. The interactions of isolated compounds with double stranded polynucleotides ctDNA and poly A – poly U (possible biological targets) were studied with circular dichroism spectroscopy (CD spectroscopy) at pH=5.0 and pH=7.0 to provide a better understanding of the biological activity of the tested compounds. Flavonoid hispidulin showed moderate antioxidant activity measured by the ABTS method while other isolated compounds from ethanolic extract showed weak antioxidant activity. Two sesquiterpene lactones (3aR, 4S, 6aR, 8S, 9aR, 9bR)-[Dodecahydro-8-dihydroxy-3, 6, 9-tris(methylene)-2oxo-2(3H)-azuleno[4, 5- b]furanyl]-3- methyl-butanoate) and hemistepsin A showed significant antibacterial activity against Staphylococcus aureus ATCC25923 (MIC 31.3 g mL-1). All tested compounds showed weak antibacterial activity against Acinetobacter baumannii DURN. Among the isolated compounds, only (3aR, 4S, 6aR, 8S, 9aR, 9bR)-[Dodecahydro- 8-dihydroxy-3, 6, 9-tris(methylene)-2oxo- 2(3H)-azuleno[4, 5-b]furanyl]-3-methyl- butanoate) exerted selective and prominent tumour cell-growth inhibitory activity towards SCCVII cell line (IC50 = 2.55μM). Simultaneously, (3aR, 4S, 6aR, 8S, 9aR, 9bR)- [Dodecahydro-8-dihydroxy- 3, 6, 9- tris(methylene)-2oxo-2(3H)-azuleno[4, 5- b]furanyl]-3-methyl-butanoate) showed a weak interaction with DNA indicating that some other target/mechanism is responsible for its prominent cytotoxic activity towards SCCVII cell line.
biological activity ; flavonoid ; sesquiterpene lactones interactions with polynucleotides
Naziv projekta: Biološka aktivnost fitokemikalija biljne vrste Centaurea ragusina L.(BioFitoCen) Šifra projekta: HR.3.2.01-0290 Projekt sufinancira Europska unija: iz Europskog socijalnog fonda u sklopu programa Razvoj ljudskih potencijala 2007. – 2013.
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Podaci o prilogu
42-42.
2016.
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objavljeno
Podaci o matičnoj publikaciji
Arhiv za higijenu rada i toksikologiju
Durgo, Ksenija
Zagreb: Institut za medicinska istraživanja i medicinu rada
0004-1254
1848-6312
Podaci o skupu
The 5th Croatian Congress of Toxicology with International Participation, CROTOX 2016.
poster
09.10.2016-12.10.2016
Poreč, Hrvatska