engleski

Investigation of Mutagenic Effect of Metals in Plomin Bay Sediments by Modified Preincubation Ames Assay.

Disposial of the dregded material from the harbour to the sea bottom represents a route throught which many contaminanats could be trabsported towards the open sea and enter to the food chain. In this study, five sediment samples were taken from different dephts and locations within Plomin bay (Istria Peninsula, Northern Adriatic Sea. Croatia).Water sediment extract were prepared and analysed for chemical and physical properties, and subjected to cytotoxic and mutagenic investigation. We used two strain of salmonella typhimurium, Ta98 (determination of frameshift) and TA100 (detection of bace pair substitution) as a test system. result showed that concentrations of heavy metals measured in extracts represent exchangeable fraction and are 500-1000 times smaller than the content of the same elements in solid mud. Nine elements were determined in extract: V, Cr, Mn, Fe, Ni, Cu, Zn, As and Pb. None of sediment sample extracts were cytotoxic or mutagenic for S. typhimurium TA98 nor TA100 in presence absence of metabolic activation.

Ames assay; Genotoxicity; Heavy metals; Marine sediments

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