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Bioinspired tripeptide hydrogels as potential biomaterials in tissue engineering

Hydrogel biomaterials, closely mimicking the three-dimensional extracellular matrix, are considered to be an ideal material for cell and tissue scaffolding applications. The dynamic nature of the noncovalent hydrogels fibril network allows the hydrogel material to spontaneously adjust to the surrounding environment and the cells to migrate through the matrix comparing to covalent hydrogels whose pores are chemically constrained and relatively inflexible.[1, 2] A new type of such supramolecular hydrogel has been designed and synthesized. The self-assembling tripeptide hydrogelator Ac-FFA-NH2 was prepared using classical methods of solution-state peptide synthesis. Prepared peptide-type hydrogelator forms fibrils by self-assembly giving stable hydrogel. Gel morphology was determined by electronic microscopy. Series of experiments have been performed in order to assess in vitro biocompatibility of the novel material. HEK293T cells have been used as a reproducible model cell line and were cultured following established proliferation protocols. Results showed high viability and high proliferation rate of the cells distributed throughout the supramolecular hydrogel structure compared to Matrigel. The obtained material was proved to serve as stabile and biocompatible physical support in improving HEK293T cells biological outcome in vitro. Furthermore, these results suggest further evaluation of in vitro biocompatibility and bioactivity on the neural stem cells upon encapsulation in order to be investigated for possible brain tissue engineering application.

tripeptide ; hydrogel ; biomaterial

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