engleski

Non-random genome distribution of translocations formed after exposure to gamma radiation:the need for standardization of the FISH method in biodosimetry

The study of chromosome translocations in relation with chromosome DNA-content after occupationla exposure to gamma radiation during non-destructive testing in industry. Eight subjects occupationally exposed to gamma radiation were analysed using fluorescent in situ hybridization (FISH) for chromosomes 1, 2 and 4. Distribution of translocations showed that chromosome 4 exhibited significantly the higher frequency of tranlsocations while chromosome 2 was significantly underrepresented respecting thier DNA content. Distribution of translocations in chromosome 1,2 and 4 are not in correlation with DNA content after occupational exposure to gamma radiation. Results of the study are in correlation with similar in vivo and in vitro studies. Non-random involvement of chromosomes point the necessity for standardization of chromosome sets in biodosimetry based on calculation of the genome equivalent

biodosimetry; FISH; standardization

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