Micronucleus, cell-free DNA and plasma glycan composition in the newborns of healthy and diabetic mothers (CROSBI ID 236955)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Fučić, Aleksandra ; Guszak, Vedrana ; Keser, Toma ; Wagner, Jasenka ; Juretić, Emilija ; Plavec, Davor ; Stojković, Ranko ; Gornik, Olga ; Lauc, Gordan
engleski
Micronucleus, cell-free DNA and plasma glycan composition in the newborns of healthy and diabetic mothers
Diabetes is associated with certain environmental exposures, heritable factors, and metabolic conditions of intrauterine development due to diabetes in the mother. We evaluated genomic damage, cell-free DNA, N-glycosylation of umbilical cord plasma proteins (PG), and nuclear division index (NDI) as possible prognostic biomarkers of health risk in the newborns of mothers with treated pregestational diabetes(NBDM ; 22 mothers), compared these parameters with those from newborns of healthy mothers (NBHM ; 89 mothers), and associated the results with the mothers’ lifestyle in both groups, based on a detailed questionnaire. Genomic damage was estimated by the in vitro micronucleus (MN) assay. NDI was detectedon MN slides. Glycans were analyzed by ultra-performance liquid chromatography that separates theplasma N-glycome into 46 glycan peaks. Cell-free DNA was analyzed by real-time PCR. For the association between biomarkers and individual characteristics, generalized linear/nonlinear analysis was performed. No significant difference was found between NBHM and NBDM for cell-free DNA levels. There was no association between cell-free DNA levels and lifestyle. MN frequency was significantly higher in NBDM than in NBHM (median, 0.6 vs. 0.3%, p < 0.001). MN frequency and NDI were significantly associated withresidence (urban vs. rural). PG differed significantly between NBHM and NBDM (p < 0.001). A significant association was found between PG and increase of MN frequency (p < 0.001). As both MN frequency and altered N-glycosylation are associated with cancer risk, our study indicates need for further investigations.
Diabetes ; Micronucleus assay ; Newborn ; Cell-free DNA ; Glycan
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Podaci o izdanju
815
2017.
6-15
objavljeno
1383-5718
10.1016/j.mrgentox.2017.01.002
Povezanost rada
Temeljne medicinske znanosti, Kliničke medicinske znanosti, Biologija