engleski

THE USE OF DIFFERENT EXTRACTION METHODS OF BIOACTIVE COMPONENTS IN LINDEN FLOWER

Linden (Tilia cordata) is abundantly used in traditional medicine worldwide due to its extensive range of positive effects on human health. Active compounds in the linden flowers include flavonoids (primarily rutin and isoquercitrin), mucilage components, tannins, procyanidins, phenolic acids and essential oils. The type, content, and proportion of these substances may vary depending on environmental factors in areas where the plants are grown. Although the usage of linden is widespread, scientific studies involving evaluation of therapeutic utilization is limited. A part of our research includes isolation of easily volatile bioactive compounds of linden flower using two methods - classical extraction (refluxing) and Soxhlet extraction. The obtained extracts is purified applying preparative thin layer chromatography/ column chromatography and follows characterization of bioactive compounds using IR - spectroscopy. Results of investigation indicate the different content of bioactive components, depending on the solvent used for extraction. Increased content of phenolic compounds was obtained while using binary polar solvents at high temperatures and classical extraction gave a higher amount of phenolic acids compared to Soxhlet extraction. Mass concentration of total phenol compounds in extract obtained employing classical extraction is 125.9 mg GAE/100 g of linden likewise 38.82 mg GAE /100 g of linden using Soxhlet extraction.

bioactive components, extraction, IR-spectroscopy, linden, total phenols

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