engleski

Development and validation of gas chromatographic-mass spectrometric method for the analysis of homogentisic acid in strawberry tree (Arbutus unedo L.) honey

In order to confirm the botanical origin of strawberry tree (Arbutus unedo L.) honey, a liquid-liquid extraction followed by gas chromatography-mass spectrometry (GC-MS) was developed for quantitative determination of homogentisic acid (HGA), the main phenolic compound in this honey. Different parameters affecting extraction, such as types and volumes of extraction solvents, pH of the solution, and salt amount were optimised. The method showed good linearity (r2 = 0.9990) over the tested concentration range (50-500 mg/kg) and low limit of detection (0.3 mg/kg). Precision expressed as relative standard deviation was <7%. The average accuracy was 95%. The optimised method was applied for determining HGA content in strawberry tree honey samples from Croatia. The HGA content in analysed samples (N = 7) ranged from 245.1 to 485.9 mg/kg. The proposed method provided reliable performance and can be easily implemented for the routine monitoring of HGA in strawberry tree honey in order to assure honey quality control.

strawberry tree honey ; Arbutus honey ; homogentisic acid ; chemical marker ; gas chromatography/mass spectrometry

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