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The microcalorimetry and force spectroscopy studies of myricetin and myricitrin interactions with model and neuronal cell membranes

Flavonoids, polyphenolic biomolecules with antioxidative activity, have recently emerged as potential novel therapeutics for neurodegenerative diseases. In order to gain more insight in the mechanisms of their protective activity, interactions of model flavonoids, myricetin [1] and myricitrin [2], with combination of microcalorimetry and force spectroscopy before and after membranes exposure to the oxidative stress. Model membranes phosphatidylcholine, sphinogomyelin and cholesterol (PC/SM/Chol), while P19 neurons were chosen to work with in order to test their ability to inhibit lipid peroxidation in model membranes and neurons in vitro. 2 3 model and neuronal cell membrane have been investigated by were made of a lipid mixture of unsaturated By microcalorimetry, local altered nanomechanical properties and structural reorganization of membrane as well as the changes within the cytoplasm, cell nucleus, and particularly cytoskeleton components induced by peroxidation were recorded. Obtained results pointed that the time needed to complete the oxidative reaction might be considered as the measure of flavonoid protective power. The nanomechanics (elasticity), surface topography (roughness) of model lipid membranes and P19 neurons as well as the thermodynamic and kinetic data that result from the oxidative damage are quantified for the first time. This highlights the potential of combining AFM, FS and microcalorimetry in elucidating the mechanism of such a complex interactions.

oxidative stress ; membrane ; flavonoids

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