Construction of Saccharomyces cerevisiae hybrid diploids for bioethanol production (CROSBI ID 647516)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Žunar, Bojan ; Pranklin, Andrea ; Lončar, Ana ; Nestić, Davor ; Svetec Miklenić, Marina ; Štafa, Anamarija ; Šantek, Božidar ; Svetec, Ivan- Krešimir
engleski
Construction of Saccharomyces cerevisiae hybrid diploids for bioethanol production
Bioethanol production from raw lignocelullosic material requires a strain of a microorganism which tolerates high ethanol concentrations and presence of growth inhibitors. Therefore we constructed hybrid diploid S.cerevisiae strains by mating two haploids having desirable traits. One haploid was isolated from wine thus tolerating high ethanol concentration and the other has been shown to be resistant to several growth inhibitors. These haploids were mated and resulting homozygous and heterozygous (hybrid) diploids were tested for the ability to produce ethanol and to grow in the presence of several growth inhibitors frequently found in different lignocelullosic hydrolysates (acetic and levulinic acid, 2-furaldehyde). The results showed that some hybrid diploids were more resistant to tested inhibitors than parental haploid strains and homozygous diploids. It is also interesting that constructed strains showed different fermentation ability in CO2 production test, suggesting genetic variability of constructed hybrids. All together, these results suggest that the approach used in this study may help to construct new strains as well as to improve traits of biotechnologically interesting strains.
hybrid diploids, bioethanol production
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Podaci o prilogu
2016.
objavljeno
Podaci o matičnoj publikaciji
EBTNA 2016
Riga:
Podaci o skupu
EBTNA 2016
poster
05.05.2016-07.05.2016
Riga, Latvija