engleski

Assessment of chemotherapy-induced DNA damage in peripheral blood leukocytes of cancer patients using the alkaline comet assay

The alkaline comet assay was employed to assess the pre- and post-treatment Ievels of in vivo DNA damage in peripheral blood Ieukocytes of twelve cancer patients. During study all patients were given antineoplastic drugs, mainly as polychemotherapy. To quantify the DNA damage the comet taillength and the tail moment were evaluated. Our results indicate marked interindividual variations between baseline DNA damage in peripheral blood Ieukocytes recorded among cancer patients prior to the chemotherapy. After intravenous administration of various antineoplastic drugs a significantly increased Ievel of DNA damage in all cancer patients compared to their pre-treatment values was recorded. The highest Ievel of DNA damage was pronounced in a patient received antineoplastic drugs according to COPP/ABV protocol. Despite of their Iimitations, our results confirm the usefulness of the alkaline comet assay as a sensitive biomarker of exposure that enables rapid and simple detection of primary DNA damage in peripheral blood Ieukocytes of cancer patients. Together with standard cytogenetic endpoints comet assay provides a powerful technique for the routine detection of critical DNA Iesions produced after administration of antineoplastic drugs in the clinical settings.

comet assay; Ieukocytes; antineoplastic drugs; interindividual variations; DNA damage

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