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The effect of boron on growth and development in strawberry (Fragaria ananassa Duch.) shoot culture

The effect of boron was tested on three strawberry (Fragaria ananassa) cultivars: Korona, Elsanta and Gorella. Boron was added into the culture medium as boric acid (H3BO3) with the concentration ranging from 0.0 to 8.68 mg B L-1, and the medium was solidified with 0.8% agar. A liquid medium without B was tested, also. The tested B concentration did not affect the time of shoot appearance but affected the number of newly developed shoots at the multiplication phase of culture. Regardless of the B quantity added to the medium in the rooting phase, the time of appearance and the number of rootlets did not differ in the same cultivar. Strawberry plantlets in axenic culture were tolerant of high B quantities in a medium, but they also survived in a medium with no boron added. Increase of B concentration to eight times the amount in MS (Murashige and Skoog) nutrient medium did not provoke necrosis nor inhibit shoot growth. The B concentration in plantlets reflected the increase of B concentration in the medium.

Boron, Fragaria sp., in vitro culture, roots, shoots, strawberry

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