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Augmentation of NKT and NK cell-mediated cytotoxicity by peptidoglycan monomer linked with zinc (CROSBI ID 95678)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Mrakovčić-Šutić, Ines ; Radošević-Stašić, Biserka ; Šimin, Marija ; Muhvić, Damir ; Rukavina, Daniel Augmentation of NKT and NK cell-mediated cytotoxicity by peptidoglycan monomer linked with zinc // Mediators of inflammation, 11 (2002), 2; 129-135-x

Podaci o odgovornosti

Mrakovčić-Šutić, Ines ; Radošević-Stašić, Biserka ; Šimin, Marija ; Muhvić, Damir ; Rukavina, Daniel

engleski

Augmentation of NKT and NK cell-mediated cytotoxicity by peptidoglycan monomer linked with zinc

BACKGROUND: Peptidoglycan monomer (PGM), which was originally prepared by biosynthesis from culture fluids of penicillin-treated Brevibacterium divaricatum, is an mmunostimulator, the activities of which might be improved by addition of zinc (Zn) to the basic molecule. METHODS: To test the possible cytotoxic effects of this new analogue, we analyzed the ability of PGM-Zn and PGM to change the phenotypic profile of hepatic and splenic mononuclear lymphatic cells and to affect the growth of malignant T-cell line YAC-1 and syngeneic thymocytes. RESULTS: Pretreatment of C57BL/6 mice primarily with PGM-Zn over 6 days (10/mg/kg ntraperitoneally) significantly enhanced the proportions of NK1.1high+, CD4-CD8-, CD69+, and CD3intermediate/NK1.1+/IL2R-beta+ (NKT) cells in the liver, and major histocompatibility complex class II+, CD69+, and CD8+ cells in the spleen. Both types of cells were highly cytotoxic against YAC-1 and syngeneic thymocytes, increasing the destruction of YAC-1 by 70% on addition of hepatic cells and by 30% on addition of splenic cells. Destruction of thymocytes increased by 10 and 50%, respectively. CONCLUSION: The results point to PGM-Zn as a potent cytotoxicity-inducing agent, which also generates autoreactive NKT cells.

NKT cell; liver; cytotoxicity; peptidoglycan monomer linked with zinc

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Podaci o izdanju

11 (2)

2002.

129-135-x

objavljeno

0962-9351

Povezanost rada

Temeljne medicinske znanosti, Veterinarska medicina, Farmacija

Indeksiranost