A single nucleotide polymorphism of DNA methyltransferase 3B gene is a potential risk factor for reccurent spontaneous abortion (CROSBI ID 650421)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Barišić, Anita ; Pereza, Nina ; Hodžić, Alenka ; Peterlin, Borut ; Ostojić, Saša
engleski
A single nucleotide polymorphism of DNA methyltransferase 3B gene is a potential risk factor for reccurent spontaneous abortion
Aim: Recurrent spontaneous abortion (RSA) is defined as three or more consecutive pregnancy losses before the 22nd week of gestation. It affects approximately 1% of couples and can be caused by several factors. However, the cause remains unidentified in about 50% of the cases, which are classified as idiopathic (IRSA). Among various possible etiological factors, aberrant DNA methylation has been suggested to be one of the possible causes of IRSA. Considering the growing evidence of the important roles of DNA methylation in gametogenesis and early pregnancy, as well as the results of multiple studies that indicate abnormal methylation patterns in the endometrium, spermatozoa and placenta of patients with IRSA, our aim was to investigate the potential association of DNA methyltransferase gene polymorphisms (DNMT1 rs2228611, DNMT3A rs1550117 and DNMT3B rs1569686) with IRSA in Slovenian reproductive couples. Patients and methods: 146 couples with ≥3 idiopathic spontaneous abortions and 149 control women and men were included in this case-control study. Genotyping was performed using polymerase chain reaction and restriction fragment length polymorphism methods. Results: We found a statistically significant higher frequency of the DNMT3B rs1569686 GG genotype (X2=7.37, P=0.025) and G allele (X2=6.33, P=0.012) in women with IRSA compared to controls. Additionally, the odds for IRSA in women were increased under the recessive genetic model (GGvsTG+TT: OR=1.92 ; 95% CI=1.18-3.09 ; P=0.008). There were no statistically significant differences in genotype and allele frequencies of any other tested polymorphism between IRSA patients and controls. Moreover, no significant associations occurred between the DNMT1 rs2228611 and DNMT3A rs1550117 polymorphisms and the risk of IRSA. Conclusion: Our results suggest that the GG genotype of the rs1569686 polymorphism in the DNMT3B gene in women might be a genetic marker for IRSA.
DNA methyltransferases ; pregnancy ; recurrent spontaneous abortion ; single nucleotide polymorphisms
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o prilogu
226-226.
2017.
objavljeno
Podaci o matičnoj publikaciji
The Tenth ISABS Conference on Forensic and Anthropologic Genetics and Mayo Clinic Lectures in Individualized Medicine
Ordog, Tamas
Zagreb: Grafički zavod Hrvatske
978-953-57695-2-1
Podaci o skupu
The Tenth ISABS Conference on Forensic and Anthropologic Genetics and Mayo Clinic Lectures in Individualized Medicine
poster
19.06.2017-24.06.2017
Dubrovnik, Hrvatska