engleski

DETERMINATION OF CARBOHYDRATES AND AMINO ACIDS IN COMPLEX MATRICES

Ion chromatography is one of the most intensively used methods for analysis of biomolecules such as amino acids and carbohydrates. Generally, this includes gradient separation on anion exchange resin followed by pulse amperometric detection. Gradient and pulse profile has to be optimized in order to obtain good quality results. Presence of the natural matrix makes method development even more complex process. This lecture review and summarizes current methods for the analysis of amino acids and carbohydrates. Several examples will be presented including fermentation broth, cell cultures and tea revealing key factors within method development process.

ion chromatography ; carbohydrates ; amino acids

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