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Basophil activation test in diagnosis and monitoring of hymenoptera venom allergies in children: in vitro measurement of basophil activation and/or degranulation (CROSBI ID 650775)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija

Zenić, Lucija ; Polančec, Denis ; Živkovi , Jelena ; Bulat Lokas, Sandra ; Turkalj, Mirjana Basophil activation test in diagnosis and monitoring of hymenoptera venom allergies in children: in vitro measurement of basophil activation and/or degranulation // 32nd Congress of the International Society for Advancement of Cytometry : abstracts. 2017. str. xx-xx

Podaci o odgovornosti

Zenić, Lucija ; Polančec, Denis ; Živkovi , Jelena ; Bulat Lokas, Sandra ; Turkalj, Mirjana

engleski

Basophil activation test in diagnosis and monitoring of hymenoptera venom allergies in children: in vitro measurement of basophil activation and/or degranulation

The diagnosis of insect venom allergy and the indication for specific immunotherapy is mostly based on skin prick test (SPT), serum specific IgE (sIgE) and patient medical history. However, given a diagnostic error of specific IgE of about 20-30%, and no real predictive value of sIgE and SPT after immunotherapy, there is a growing need for an in vitro method that would allow a better understanding of allergic reactions and help clinicians in cases where other diagnostic procedures give ambiguous results. We represent an improved basophil activation test (BAT) developed to monitor basophil activation and degranulation by a simultaneous analysis of the surface expression of CD63 and CD203c using flow cytometry. Objectives were to test clinical utility of an improved BAT in the diagnosis and management of hymenoptera venom allergies as a daily routine in our hospital, i.e. to test the CD203c basophil activation marker and its correlation with CD63 after hymenoptera venom allergen stimulation. Methods: Samples of peripheral blood of children allergic to hymenoptera venom were prepared using the FlowCAST® (Bühlmann, Switzerland) protocol with the addition of the antibodies against CD45 and CD203c. Acquistion of the samples was performed using the Navios flow cytometer (Beckman Coulter, USA) and the data were analysed using the FlowLogic™ software (Inivai Technologies, Australia). For both markers the percentage of activated basophils and the mean fluorescence intensity (MFI) was determined in parallel. The addition of the antibodies against CD45 and CD203c to a commonly used CCR3/CD63 antibody combination enabled a more precise basophil identification. The MFI of CD203c differed among samples treated with different hymenoptera venom allergen concentrations in a gradient concentration-manner, although the percentage of activated basophils estimated by CD63 expression did not differ between those samples. Our results indicate that the differences in CD203c expression are measurable even during a non-degranulating stimulation of basophils. The use of anti-CD203c antibody, in combination with the antibodies against CD45, CD63 and CCR3, enabled a better resolution of the activated/non-degranulated and activated/degranulated basophils. The use of CD203c improved both the sensitivity and the specificity of BAT, strengthening its potential in the diagnosis of hymenoptera venom allergies and in discrimination between sensitized children and children with developed allergy to hymenoptera venom.

BAT, IgE

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Podaci o prilogu

xx-xx.

2017.

nije evidentirano

objavljeno

Podaci o matičnoj publikaciji

32nd Congress of the International Society for Advancement of Cytometry : abstracts

Podaci o skupu

32nd Congress of the International Society for Advancement of Cytometry (32 ; 2017)

poster

10.06.2017-14.06.2017

Boston (MA), Sjedinjene Američke Države

Povezanost rada

Kliničke medicinske znanosti