engleski

Profiles of trace elements associated to biomolecules in hepatic cytosols of two freshwater fish species

The aim of this study was to define and compare the distributions of five trace elements (essential: Cu, Fe, Se, Zn ; non-essential: Cd) among cytosolic biomolecules of different molecular masses isolated from livers of European chub (Squalius cephalus) and brown trout (Salmo trutta). These two fish species are often applied as bioindicators of environmental contamination in different types of fluvial ecosystems. European chub were sampled in the lowland river Sutla and brown trout in the karstic river Krka in Croatia, in autumn seasons of 2009 and 2015, respectively. To obtain the elemental profiles, metals bound to cytosolic biomolecules were separated by size exclusion high performance-liquid chromatography and subsequently analysed by high resolution-inductively coupled plasma-mass spectrometry. Obtained profiles indicated certain differences between two species. Both Cd and Cu were associated to metallothioneins in both species, but only in trout their binding to high molecular mass biomolecules (>100 kDa) was evident. Binding of Fe and Se to certain very low molecular mass biomolecules (<10 kDa) was observed only in trout, whereas distribution profiles of Zn were comparable in both studied species. The results of this study provide the possibility for better understanding of metal/metalloid fate in the cells.

metals ; metalloid ; size exclusion chromatography ; ICP-MS ; European chub - Squalius cephalus ; brown trout - Salmo trutta

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