engleski

Monitoring of selected pharmaceuticals in surface waters of Croatia

Sulfonamides, macrolides, torasemide, fumagillin and chloramphenicol were simultaneously analyzed in surface water samples by using solid-phase extraction (SPE) and reversed phase (RP) liquid chromatography- electrospray tandem mass spectrometry (LC-ESI- MS/MS). In the pre-concentration and clean-up process the pH value of samples and volume of the solvent for extraction of analytes from cartridge were optimized. Extraction recoveries were high with values in the range from 62 to 115 %. Limits of quantification (LoQ) were in the range from 0.02 g L–1 to 0.2 g L–1. Repeatability of the method was evaluated at LoQ and expressed as relative standard deviation (RSD). Calculated RSDs were low with values in the range from 2.4 to 14.5 %. The method was successfully applied for analysis of surface water real samples. Samples were collected along the rivers in Croatia on 4 sampling sites in 2012 in Danube catchment areas, 19 sampling sites in Danube and Adriatic catchment areas in 2013, and another 19 places in 2014. Altogether, 20 target compounds were analysed in 164 water samples and detected in 24 samples in range (0.1–5.3) g L–1 or in 14.6 % of samples. The most frequent and highest concentrations were detected for macrolide antibiotics. This is the first attempt of such monitoring in surface waters in Croatia.

sulfonamides, macrolides, surface waters, environment, LC-MS/MS analysis

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