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Acute exposure to hyperbaric oxygenation impaires endothelial nitric oxide production in Sprague-Dawley healthy male rats (CROSBI ID 651471)

Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija

Jukić, Ivana ; Mihaljević, Zrinka ; Rašić, Lidija ; Ćosić, Anita ; Stupin, Ana ; Ezgeta, Ivana ; Drenjančević, Ines Acute exposure to hyperbaric oxygenation impaires endothelial nitric oxide production in Sprague-Dawley healthy male rats // Acta physiologica. 2017. str. 126 (B02-9)-x

Podaci o odgovornosti

Jukić, Ivana ; Mihaljević, Zrinka ; Rašić, Lidija ; Ćosić, Anita ; Stupin, Ana ; Ezgeta, Ivana ; Drenjančević, Ines

engleski

Acute exposure to hyperbaric oxygenation impaires endothelial nitric oxide production in Sprague-Dawley healthy male rats

Question: Previously, study from our laboratory showed that acute exposure to hyperbaric oxygenation (HBO) increase oxidative stress production. This increased oxidative stress caused impaired endothelium-dependent vasodilation while superoxide scavenger TEMPOL in vitro restored that NO-dependent dilation. Present study aimed to determine the effects of acute HBO exposure on flow-induced endothelial NO production. Methods: Healthy male 11 weeks old Sprague-Dawley rats were divided in: CTRL (control group, N=5) and A-HBO (acute single time exposure to HBO, N=5). Exposure to HBO was in hyperbaric oxygen chamber (100% oxygen ; 2 bar/2 hours). Prior to decapitation rats were anesthetized with ketamin and midazolam. Middle cerebral arteries were isolated and cannulated and pressurized at ∆80 mmHg with flow on, on pressure myograph on, in the absence/presence of TEMPOL in vitro. NO production was determined by DAF-2DA to DAF-2T conversion fluorescence assay. All experimental procedures conformed to the European Guidelines for the Care and Use of Laboratory Animals (directive 86/609) and were approved by institutional Ethical Committee. Results: Flow-induced NO production was significantly lower in A-HBO group compared to flow-induced NO production in CTRL group (p=0.020). TEMPOL in vitro increased endothelial NO production in A-HBO group, compared to A-HBO in the absence of TEMPOL (p=0.011). NO production in control group was similar with/without TEMPOL in vitro (p=0.62) suggesting low level of oxidative stress in control rats. Conclusion: Acute exposure to HBO increases oxidative stress. Superoxide scavenging by TEMPOL restores flow-induced NO production in A-HBO group. Funding: research grant VIF2016-MEFOS-19

hyperbaric oxygenation, nitric oxide, endothelium, Sprague-Dawley rats

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Podaci o prilogu

126 (B02-9)-x.

2017.

nije evidentirano

objavljeno

Podaci o matičnoj publikaciji

Acta physiologica

1748-1716

Podaci o skupu

FEPS 2017

poster

13.09.2017-15.09.2017

Beč, Austrija

Povezanost rada

Temeljne medicinske znanosti

Indeksiranost