engleski

Oxidative stress in animals treated with ochratoxin A and citrinin and its reversal by resveratrol

Ochratoxin A (OTA) and citrinin (CTN) are nephrotoxic mycotoxins that often contaminate food and feed. The mechanisms of both mycotoxins include oxidative stress, and the aim of this study was to evaluate the effects of combined exposure to these two mycotoxins on oxidative stress in experimental animals and the counterfeits of resveratrol (RSV). Adult male Wistar rats were divided in the following groups (n=5 each) to orally receive: water (controls), solvent (NaHCO3), OTA(0.125 mg kg-1 b.m.), OTA(0.250 mg/kg b.m.), CTN(2.0 mg/kg b.m.), OTA(0.125 mg)+CTN(2.0 mg), OTA(0.250 mg)+CTN(2.0 mg), and OTA(0.250mg)+CTN(2.0 mg)+RSV(20 mg). In this study malondialdehyde (MDA) and glutathione (GSH) in the plasma, kidney and liver, and the catalytic activity of glutathione peroxidase (GPx) in the plasma were measured. MDA levels were significantly increased in the kidney of animals receiving either of the OTA doses alone (34.21±3.90 and 41.81±5.51 ng/g tissue, respectively) as well as in animals receiving OTA (0.125) + CTN (45.07±12.59). Resveratrol did not reverse these effects in the kidney (23.96±1.83). In the plasma both OTA doses and CTN increased MDA levels (0.68±0.08, 0.65±0.05, 0.65±0.09 ng/mL), but not the combined treatment (0.69±0.07). This time, however, RSV decreased it significantly (0.43±0.03). RSV was also effective in the kidney, having restored its GSH levels. GPx activity in plasma was significantly lower only in animals treated with the lower OTA dose + CTN. Parameters of oxidative stress are not equally affected by OTA + CTN treatment, and RSV does not always reverse their effects.

53rd Congress of the European Societies of Toxicology (EUROTOX)

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