engleski

Morphometrical evaluation of histopathological alterations in freshwater snail Holandriana holandrii exposed to phenol

Morphometrical observations of histopathological changes induced by phenol intoxication were performed and analysed on the freshwater snail Holandriana holandrii. We exposed animals to sodium pentachlorophenate (Na-PCP ; 0.56, 1.0 mg/L) through the period of acute toxicity, and compared them with the control samples. Morphometrical analysis of target organs were performed on routine paraffine sections using light microscope with a digital camera AxioCam ERc 5s and software Zen 2 Lite (Carl Zeiss Microscopy GmbH, Germany). Measurement was conducted on tubular area, tubular lumen area and vacuoles of digestive tubules and epithelium and lamellar width of the gills. We recorded and compared remarkable histopathological alterations in animals treated with toxicant through the time and between different concentrations, which included shedding and swelling of tubular cells and disorganization of tubules in digestive glands, while lamellar spaces in the gills were distended. Histomorphometry was confirmed as a powerful tool in the quantitative measurements of ecotoxicological impact of xenobiotics upon freshwater organisms.

hepatopancreas, gills, freshwater molluscs

nije evidentirano