engleski

Acinetobacter baumannii survive anaerobic digestion of sewage sludge.

Multi-drug resistant (MDR) Acinetobacter baumannii were found in untreated as well as in biologically or chemically treated hospital and municipal wastewaters. However, there is no evidence about the fate of A. baumannii in the wastewater treatment process. The aim of this study was to screen the sewage sludge after its stabilization by anaerobic mesophilic digestion for the presence of A. baumannii. On 9 sampling occasions at the municipal wastewater treatment plant of the City of Zagreb, 17 isolates of A. baumannii were recovered form digested sludge with MALDI-TOF MS score values ranging from 2.026-2.288. 3/17 isolates were sensitive to all antibiotics, while 14 MDR isolates shared the resistance to carbapenems and fluoroquinolones but sensitivity to colistin. Technology of anaerobic sludge digestion was performed at 36°C, neutral pH and digestion time of 21-36 days. In laboratory controlled anaerobic conditions isolates were able to survive on Nutrient agar in anaerocultA system during 30 days, after which multiplied normally in aerobic conditions. However, isolates were not able to multiply directly in anaerocultA. The finding confirms the need of proper management and disposal of sewage sludge generated at wastewater treatment plants in order to prevent the spread of MDR A. baumannii in nature.

environment, public health, wastewater treatment plant, sewage sludge treatment

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