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izvor podataka: crosbi

Potential use of apple polyphenol oxidase for bioremediation of phenolic contaminants (CROSBI ID 242837)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Šalić, Anita ; Strelec, Ivica ; Zelić, Bruno ; Tišma, Marina Potential use of apple polyphenol oxidase for bioremediation of phenolic contaminants // Kemija u industriji : časopis kemičara i tehnologa Hrvatske, 67 (2018), 3-4; 109-116. doi: 10.15255/KUI.2017.036

Podaci o odgovornosti

Šalić, Anita ; Strelec, Ivica ; Zelić, Bruno ; Tišma, Marina

engleski

Potential use of apple polyphenol oxidase for bioremediation of phenolic contaminants

Phenolic compounds, such as catechol, are released into the environment from a variety of industrial sources and they present serious ecosystem burden. This work examined possibility to use partially purified apple polyphenol oxidase (PPO) for bioremediation of phenolic contaminants. In order to optimize process conditions, optimal pH and temperature for PPO activity were determined, while PPO affinity toward various phenols, as well as effect of some salts and organic solvents which can be found in wastewaters, was used to confirm applicability of PPO in wastewater treatment. It was found that partially purified apple PPO shows maximal activity at pH 6.8 and 25 °C, but exhibit more than 85 % of its maximal activity in pH range from 5 to 8, and more than 90 % of activity in temperature range from 10 to 50 °C. PPO showed high affinity for various di-phenols, but lack of affinity toward mono-phenols. Sodium tetraborate decahydrate moderately inhibited PPO activity, while exposure of PPO to the presence of organic solvents ( = 5 %) caused 40 % loss of its activity. Catechol oxidation by PPO performed for just 5 min in a batch reactor at optimal process conditions resulted in 25 % conversion . Based on obtained data, it seems that partially purified apple PPO has reasonable potential in wastewater treatment.

catechol oxidation ; enzymatic wastewater treatment ; phenolic contaminants ; polyphenol oxidase (PPO)

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Podaci o izdanju

67 (3-4)

2018.

109-116

objavljeno

0022-9830

1334-9090

10.15255/KUI.2017.036

Povezanost rada

Biotehnologija

Poveznice