Design of Small Molecular Probes for Proteins and DNA / RNA (CROSBI ID 652986)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa
Podaci o odgovornosti
Piantanida, Ivo
engleski
Design of Small Molecular Probes for Proteins and DNA / RNA
Small molecules targeting DNA, RNA and/or proteins have attracted significant scientific interest not only because of their biomedicinal applications, but also due to widespread use of spectrophotometric markers in the related scientific research. For instance, fluorescent techniques significantly developed during the last three decades and now represent about 70% of the detection enabling technologies used in molecular biology and medicine. However, design of small molecule (Mw<600) for recognition of ds-DNA/RNA sequence or particular protein is very challenging due limited number of modifications in such restricted molecule size. Quite often, small modifications lead to change of target preference, for instance from DNA-targeting to protein-targeting molecule One of our research lines is focused on the generally under-investigated approach: exploitation of intrinsic property of some dyes for aggregation, whereby monomeric and aggregated dye differ strongly not only in target recognition but also in spectroscopic properties1. Thus, one dye molecule could bind with similar affinity to several targets (DNA, RNA, protein) giving different spectroscopic responses for each target: to some polynucleotide sequence dye would bind as monomer, for other sequence as dimer, and protein binding site would induce different spectroscopic response (Scheme 1). The ongoing research endeavours to establish for the low molecular weight dyes the structure-activity guidelines for fine tuning of DNA - RNA - protein preferences combined with recognition by set of sensitive and bio-applicable spectrometric methods.
DNA recognition ; protein recognition ; fluorescence ; CD ; aggregation
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Podaci o prilogu
41-41.
2017.
objavljeno
Podaci o matičnoj publikaciji
Book of Abstracts of the 10th Joint Meeting on Medicinal Chemistry
Basarić, Nikola ; Namjesnik, Danijel ; Perković, Ivana ; Stepanić, Višnja
Zagreb: Hrvatsko kemijsko društvo
978-953-55232-8-4
Podaci o skupu
10th Joint Meeting on Medicinal Chemistry
ostalo
25.06.2017-28.06.2017
Srebreno, Hrvatska; Dubrovnik, Hrvatska