Molecular imaging of cannabis leaf tissue with MeV-SIMS method (CROSBI ID 243660)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Jenčič, Boštjan ; Jeromel, Luka ; Ogrinc Potočnik, Nina ; Vogel-Mikuš, Katarina ; Kovačec, Eva ; Regvar, Marjana ; Siketić, Zdravko ; Vavpetič, Primož ; Rupnik, Zdravko ; Bučar, Klemen ; Kelemen, Mitja ; Kovač, Janez ; Pelicon, Primož
engleski
Molecular imaging of cannabis leaf tissue with MeV-SIMS method
To broaden our analytical capabilities with molecular imaging in addition to the existing elemental imaging with micro-PIXE, a linear Time-Of-Flight mass spectrometer for MeV Secondary Ion Mass Spectrometry (MeV-SIMS) was constructed and added to the existing nuclear microprobe at the Jožef Stefan Institute. We measured absolute molecular yields and damage cross-section of reference materials, without significant alteration of the fragile biological samples during the duration of measurements in the mapping mode. We explored the analytical capability of the MeV-SIMS technique for chemical mapping of the plant tissue of medicinal cannabis leaves. A series of hand-cut plant tissue slices were prepared by standard shock-freezing and freeze-drying protocol and deposited on the Si wafer. We show the measured MeV-SIMS spectra showing a series of peaks in the mass area of cannabinoids, as well as their corresponding maps. The indicated molecular distributions at masses of 345.5 u and 359.4 u may be attributed to the protonated THCA and THCA-C4 acids, and show enhancement in the areas with opened trichome morphology.
Imaging mass spectrometry ; MeV-SIMS ; Molecular imaging ; THC ; THCA
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Podaci o izdanju
371
2016.
205-210
objavljeno
0168-583X
1872-9584
10.1016/j.nimb.2015.10.047