engleski

Antibacterial efficacy of five bioactive root canal sealers against Enterococcus faecalis biofilm

Aim To determine the antimicrobial effect of five bioactive endodontic sealers against 48 h old Enterococcus faecalis biofilms. Methodology Bacterial suspension with E. faecalis, which had been isolated from root canal with chronic apical periodontitis, was grown on cellulose nitrate membrane filters (Whatman GmbH, Germany) and placed on the Mitis Salivarius agar plates. After the incubation period (48 h), the colonization was con- firmed by scanning electron microscopy. Then, the membranes were divided into six experimental groups (n = 8/each) according to the tested sealer: 1: TotalFill Bioceramic Sealer (BC Sealer, FKG, Switzerland) ; 2. BioRoot RCS (Septodont, Saint-Maur-Des- Fosses, France) ; 3. MTA Fillapex (Angelus, Londrina, Brasil) ; 4. MTA Plus (PrevestDenpro, Bradenton, USA) ; 5. Apexit (Vivadent, Schaan, Liechtenstein) ; 6. AH Plus (Dentsply, Konstanz, Ger- many). The sealers were prepared according to the manufactur- ers’ instructions and approximately 40 lL of each sealer was placed on each membrane. After 30 min of exposure, sealers were removed. The membranes were transferred to tubes containing sterile phosphate buffered saline (PBS) and agitated. After 10-fold serial dilution, the aliquots were plated on Mitis Salivarius agar plates. After 24 h of incubation colony forming units (CFUs) were counted. Positive controls included membranes with E. faecalis and without sealer, and sterile membranes with applied sealer served as negative controls. For the statistical analysis Mann Whitney U and Kruskal Wallis tests were used. Results All sealers reduced the number of bacteria significantly (p < 0.005). The greatest antibacterial effect was recorded in the Apexit group (p < 0.005). There were no significant differences in antimicrobial efficacy between other sealers (p < 0.001). Conclusions All tested bioactive endodontic sealers had a signifi- cant and similar antimicrobial effect against E. faecalis, however, Apexit was the most effective.

bioactive sealer ; antimicrobial ; Enterococcus faecalis

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