engleski

The impact of sun radiation on the parameters of comet assay

Since there are several confounding factors that are associated with comet assay parameters, we have decided to assess the impact of global sun radiation on comet assay tail length (TL) and tail intensity (TI). A total of 50 volunteers were retrospectively studied, based on date when blood donation was made. The study group was divided into hot period (March – August) and cold period (September – February). The groups were matched in age, gender, smoking status, and body mass index in order to minimize the impact of other possible confounders. Average global sun radiation 1 and 7 days before blood sampling in cold period was 46.58 ± 35.39 J/m2 and 63.02 ± 26.84 J/m2, respectively, compared to 109.92 ± 46.07 J/m2 and 101.76 ± 31.31 J/m2, respectively, in hot period. Accordingly, the TL and TI in cold period were lower (13.80 ± 1.61 μm and 1.26 ± 0.54 %, respectively) compared to hot period (14.91 ± 1.65 μm and 1.67 ± 0.65 %, respectively). Taken together, we have observed that the baseline levels of DNA damage were higher in volunteers donating blood in hot period compared to those who donated blood in cold period. Further studies are needed in order to examine the impact of sun radiation on comet assay parameters.

DNA damage ; biomarkers ; sun radiation ; seasonal variations

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