engleski

Surface display of proteins in microorganisms – potential applications in food control and processing

Studies of microbial cell envelopes and particularly cell surface proteins and mechanisms of their localization brought about new biotechnological applications of gained knowledge in surface display of homologous and heterologous proteins. By fusing surface proteins, or their anchoring domains with different proteins of interest their so called genetic immobilization is achieved. Hybrid proteins are engineered in a way that they are expressed in the host cells, secreted to the cell surface and incorporated into the wall/envelope moiety. In this way laborious and often detrimental procedure of chemical immobilization of the protein is avoided by letting the cells do the whole procedure. Both bacterial and yeast cells have been used for this purpose and a number of potential biotechnological applications of surface displayed proteins have been reported. Examples range from microbial whole cell biocatalysts, biosorbents, biosensors and biostimulants development to design and screening of protein and peptide libraries. When surface immobilized enzymes are used, substrates do not need to cross membrane barriers, i.e. enzymes are free to access any externally added substrate. Thus, often complex and expensive purification of the enzymes used on an industrial scale is bypassed. In addition, the multi-step transformation can be performed using microbial cells displaying different enzymes that catalyze cascade reactions. In recent years particular attention has been paid to yeast systems for surface display of proteins since most yeasts are generally regarded as safe (GRAS) microorganisms, yeast cell walls are capable of binding more proteins, and the cells are bigger. Besides, yeasts are generally more suitable for expression of proteins originating from higher eukaryotes. In this talk our current knowledge on mechanisms and molecular systems for surface display of proteins on bacterial and yeast cell surfaces will be discussed. Particularly, potential applications of surface displayed proteins in food processing, as well as for creation of sensors suitable for food control will be summarized.

surface display, genetic immobilization, bacterial envelope, yeast cell wall, food processing enzymes

nije evidentirano