engleski

Physiologic and pathogenetic significance of perforin in pregnancy, pozvano predavanje, 7th International Congress of Reproductive Immunology, New Delhi, 27.-30.listopad 1998.

Perforin (P) positive lymphocytes are present in high concentration in the metrial gland of rodent's pregnancy and first trimester human pregnancy decidua. Experiments with P deficient mice showed that P plays a major role in CTL and NK cell-mediated cytotoxicity, but is not necessary for normal pregnancy. However, mice simultaneously deficient for both cytolytic pathways (Fas L/Fas and Perforin double deficient mice-DD) are short lived and female mice are infertile. Mice homozygous for Fas-L defect, but heterozygous at the P locus and expressing one functional P allele are fertile and protected from early death. The analyses of P expression in peripheral blood lymphocytes (PBL) of nonpregnant (NP) first trimester pregnancy (FTP) and term pregnancy (TP) females showed considerable differences: 1) Proportion of P+ cells increased significantly at the end of the pregnancy compared to FTP (22% vs. 37%) ; 2) In the FTP 18% of P+ cells are CD4+ which is 3 times higher than in NP ; 3) drastic decrease of CD16+ cells in the population of P+ cells (from 45% in NP to 4% to FTP), and increase at TP (67%). In the FTP decidua CD3-CD56bright+ CD16- decidual lymphocytes (DL) are Pbright+, which was confirmed by analyzing CD3- clones. In pathological pregnancies (anembryonic pregnancy and missed abortion) both the percentage of CD4+ cells among P+ cells and the content of P/cell were 3 times lower compared to NP. Decidual adherent cells (DAC) and/or their supernatants have pivotal role in upregulation of P expression in decidual lymphocytes. These effects can be blocked by anti IL-15 antibodies. In our opinion direct and indirect (cytokine and hormone mediated) interactions between DAC and decidual lymphocytes is responsible for high level of P expression in DL.

perforin; Rodent's pregnancy; Fas L/Fas; perforin double deficinet mice-DD; peripheral blood lymphocytes; decidual adherent cells

nije evidentirano