Membrane phenotype and expression of perforin and serine esterases by CD3- peripheral blood and decidual granular lymphocyte-derived clones (CROSBI ID 485432)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Gudelj, Lea ; Christmas, Stephen E. ; Laškarin, Gordana ; Johnson, Peter M. ; Rukavina, Daniel ; Podack, Echkard R.
engleski
Membrane phenotype and expression of perforin and serine esterases by CD3- peripheral blood and decidual granular lymphocyte-derived clones
Problem: Human first-trimester pregnancy decidua was found to contain large numbers of perforin (P)-containing cells, which varied in their membrane antigen phenotype. Here we report results obtained by analyzing CD3  clones derived from human early pregnancy decidua and peripheral blood. Method: Decidual tissue was obtained from vaginal termination of first trimester normal human pregnancies. CD3- clones were generated by limiting dilution cloning, following depletion of CD3+ lymphocytes. Cell membrane phenotype was determined by flow-cytometry. Perforin was detected by FACS analysis of permeabilised cells. Serine esterases (SE) were identified by histochemical staining for BLT-esterase. Results: Cloned decidual cell populations retained the overall antigenic phenotype of freshly isolated decidual NK-like cells. All CD3  clones, either derived from decidua or from peripheral blood contained perforin. Serine esterases were present in every decidual clone analysed. Conclusions: Limiting dilution cloning allows the clear-cut analysis of homogenous subsets of decidua derived NK-like clones. The presence of large amounts of perforin in all of the CD3-clones underlines the extensive transcription of perforin gene by NK-like lymphocytes.
CD3- clones; decidual granular lymphocytes; perforin; peripheral blood lymphocytes; serine esterases
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Podaci o prilogu
62-62-x.
1996.
nije evidentirano
objavljeno
Podaci o matičnoj publikaciji
Periodicum Biologorum
Vitale, Branko
Zagreb: Hrvatsko prirodoslovno društvo
Podaci o skupu
Third international Meeting "Mechanisms in Local Immunity"
pozvano predavanje
25.09.1996-28.09.1996
Opatija, Hrvatska