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The presence and role of interleukin-18 at maternal-fetal interface (CROSBI ID 485458)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija

Sotošek Tokmadžić, Vlatka ; Tsuji, Y. ; Bogović, Tatjana ; Laškarin, Gordana ; Ćupurdija, Kristijan ; Štrbo, Nataša ; Randić, Ljiljana ; Rukavina, Daniel The presence and role of interleukin-18 at maternal-fetal interface // Godišnji sastanak HID-a 2001 : Knjiga sažetaka. Zagreb: Hrvatsko imunološko društvo, 2001. str. 3-3

Podaci o odgovornosti

Sotošek Tokmadžić, Vlatka ; Tsuji, Y. ; Bogović, Tatjana ; Laškarin, Gordana ; Ćupurdija, Kristijan ; Štrbo, Nataša ; Randić, Ljiljana ; Rukavina, Daniel

engleski

The presence and role of interleukin-18 at maternal-fetal interface

First trimester human decidual is abundantly infiltrated with uterine NK cells (uNK) of unusual phenotype (CD3-CD16-CD56bright+) that contain high amount of cytolytic protein perforin in their granules. During early pregnancy uNK cells are exposed to many cytokines that influence their cytolytic activity. Recently discovered IL-18, as a multifunctional cytokine, participates in regulation of both Th1 and Th2 responses and augment both innate and acquired immunity. IL-18 is described as potent inducer of cytolytic activity of NK cells. Since there are very few data about IL-18 expression during pregnancy, the aim of this study was to analysed the presence of IL-18 and IL-18 receptor (IL-18R) at maternal-fetal interface and the possible role of IL-18 on perforin expression and NK cytotoxicity of decidual lymphocytes (DL). Decidual tissue of first trimester pregnancy decidua was enzyme digested and decidual cells from Lymphoprep interface was stimulated overnight with IL-18 (10 ng/ml) or cultured in the medium only. Cytolytic activity of non-adherent cells (DL) against K-562 targets was measured in 2 hr PKH-26 (red) cytotoxicity assay by flow cytometry. Decidual adherent cells (DAC) were used for intracellular staining of IL-18. The number of perforin positive DL, separated from DAC, were cultured for 18 hrs with IL-18 (5, 10, 15 or 20 ng/ml) or the medium only and analysed by flow cytometry. IL-18 and IL-18R expression on the trophoblast cells was detected by immunohistology. IL-18 added in a dose of 10 ng/ml upregulates perforin protein expression and cytolytic activity of DL. Approximately 20% of DAC are IL-18 positive and this percentage could be upregulated by PMA and Ionomycin stimulation. Double labelling of DAC with anti IL-18 and anti HLA-DR monoclonal antibody showed only about 3% of cells are at the same time double positive. By immunohistology IL-18R positive cells were found on syncytiotrophoblast cell layer, interstitial tissue cells of villi and fetal blood cells. There was no detectable IL-18 staining on trophoblast cell layer on villi, but strong staining of fetal blood cells in villous vessels. These are first results showing IL-18R expression, but not IL-18 expression on villous trophoblast cells, as well as enhancement of perforin expression and NK cytolytic potential of DL under the influence of IL-18. IL-18 in concert with other cytokines and hormones could play an important role in the regulation of cytolytic potential of first trimester pregnancy decidual NK cells.

cytolytic activity; decidual lymphocytes; IL-18; perforin; IL-18R

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Podaci o prilogu

3-3.

2001.

nije evidentirano

objavljeno

Podaci o matičnoj publikaciji

Godišnji sastanak HID-a 2001 : Knjiga sažetaka

Zagreb: Hrvatsko imunološko društvo

Podaci o skupu

Godišnji sastanak HID-a

predavanje

01.01.2001-01.01.2001

Zagreb, Hrvatska

Povezanost rada

Temeljne medicinske znanosti