Nalazite se na CroRIS probnoj okolini. Ovdje evidentirani podaci neće biti pohranjeni u Informacijskom sustavu znanosti RH. Ako je ovo greška, CroRIS produkcijskoj okolini moguće je pristupi putem poveznice www.croris.hr
izvor podataka: crosbi

Interaction of progesterone and IL-15 in the regulation of perforin expression and cytolytic activity of decidual lymphocytes at the materno-fetal interface (CROSBI ID 485466)

Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija

Laškarin, Gordana ; Štrbo, Nataša ; Sotošek-Tokmadžić, Vlatka ; Bogović-Crnčić, Tatjana ; Ćupurdija, Kristijan ; Juretić, Koraljka ; Dorčić, Dorotea ; Dupor, Jana ; Randić, Ljiljana ; Rukavina, Daniel et al. Interaction of progesterone and IL-15 in the regulation of perforin expression and cytolytic activity of decidual lymphocytes at the materno-fetal interface // American journal of reproductive immunology. 2002. str. 160-160

Podaci o odgovornosti

Laškarin, Gordana ; Štrbo, Nataša ; Sotošek-Tokmadžić, Vlatka ; Bogović-Crnčić, Tatjana ; Ćupurdija, Kristijan ; Juretić, Koraljka ; Dorčić, Dorotea ; Dupor, Jana ; Randić, Ljiljana ; Rukavina, Daniel ; Podack, Eckhard R.

engleski

Interaction of progesterone and IL-15 in the regulation of perforin expression and cytolytic activity of decidual lymphocytes at the materno-fetal interface

Human first trimester pregnancy decidua, characterised by high local concentration of progesterone, is infiltrated by decidual lymphocytes (DL) and HLA-DR+ cells, mostly macrophages. Predominant lymphocyte population are decidual non-classical NK cells. These cells are CD45+CD56bright+ and contain high level of perforin (P) in their granules. IL-15, as a main NK differentiation and proliferation factor is highly expressed in decidua and secreted by HLA-DR+ cells. Our previous results showed that progesterone in high concentration downregulates the number of P+ cells, as well as the content of P per cell, while IL-15 upregulates P expression in cultured DL. The aim of this study was to investigate possible role of progesterone in regulation of IL-15 induced cytotoxicity of DL. The suspension of freshly isolated decidual cells was incubated 18 or 72 hrs with progesterone, IL-15 or combination and cytolytic activity of DL was measured against NK sensitive K562 cells in 2 hrs cytotoxicity PKH26 (red) test. Concanymicin A pre-treatment was used to assess the contribution of perforin-mediated cytotoxcitiy. Intracellular content of perforin and IL-15 was measured by flow cytometry. We found that IL-15 on a dose dependent manner increase cytolytic activity of DL. Cytolytic activity of IL-15 stimulated DL for 72 hrs against K562 cell line was almost completely abrogated by Concanamycin A. Progesterone decreases cytolytic activity of DL and the percentage of IL-15+/HLA-DR+ cells in the culture. IL-15 reverted progesterone mediated suppression of cytolytic activity of DL against K562 cells. Conclusion: Our results clearly show that progesterone affects perforin mediated cytotoxcitiy of DL, as well as expression of IL-15 by decidual HLA-DR+ cells.

first trimester pregnancy ; decidua ; perforin ; IL-15

Germany Conference venue : 'neue weimarhalle'.

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o prilogu

160-160.

2002.

nije evidentirano

objavljeno

Podaci o matičnoj publikaciji

American journal of reproductive immunology

1600-0897

Podaci o skupu

Congress of the Alps Adria Society for Immunology of Reproduction (8 ; 2002)

poster

07.09.2002-10.09.2002

Weimar, Njemačka

Povezanost rada

Temeljne medicinske znanosti

Indeksiranost