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izvor podataka: crosbi

Preanalytics of urine sediment examination: effect of tube type, volume of sample, relative centrifugation force and supernatant removal (CROSBI ID 248209)

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Bunjevac, Amalija ; Nikolac Gabaj, Nora ; Miler, Marijana ; Horvat, Anita Preanalytics of urine sediment examination: effect of tube type, volume of sample, relative centrifugation force and supernatant removal // Biochemia medica, 28 (2018), 1; 010707, 12. doi: 10.11613/BM.2018.010707

Podaci o odgovornosti

Bunjevac, Amalija ; Nikolac Gabaj, Nora ; Miler, Marijana ; Horvat, Anita

engleski

Preanalytics of urine sediment examination: effect of tube type, volume of sample, relative centrifugation force and supernatant removal

Laboratories often modify procedures recommended by the European Urinalysis Guidelines for urine sediment analysis. The aim of this study was to compare the recommended protocol with our routine laboratory procedure and to evaluate the possible impact of modifications in the relative centrifugal force, type of tube, method of supernatant aspiration and urine volume on patient’s results. Firstly, relative centrifugal force was investigated using 20 pairs of samples examined after centrifugation at 400xg and 1358xg. In phase two, 110 samples were examined, paired as: round bottom vs conical tube (N = 46), decanting vs suction of supernatant (N = 100) and 10 mL vs 5 mL of urine sample (N = 101). The number of erythrocytes, leukocytes and squamous epithelial cells was significantly lower after centrifugation at 400xg (P = 0.001, 0.002 and 0.004, respectively). The number of leukocytes was significantly lower in conical tubes (P = 0.010), after the suction of supernatant (P = 0.045) and in 5 mL urine (P < 0.001). The number of squamous epithelial cells was significantly lower after the suction of supernatant (P < 0.001) and in 5 mL urine (P < 0.001). The number of erythrocytes (P < 0.001), total non-hyaline casts (P < 0.001) and the frequency of granular casts (P = 0.039) was significantly lower in 5 mL urine. Lower results of leukocytes, erythrocytes, squamous cells and non-hyaline casts were recorded in recommended procedures (centrifugation at 400xg, suction of supernatant, conical tube, 5 mL of sample) than in routine procedure (centrifugation at 1358xg, decanting of supernatant, round bottom tube, 10 mL) used in our laboratory.

preanalytical phase ; laboratory error ; urinalysis ; urine sediment

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Podaci o izdanju

28 (1)

2018.

010707

12

objavljeno

1330-0962

1846-7482

10.11613/BM.2018.010707

Povezanost rada

Kliničke medicinske znanosti

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