engleski

An analysis of genetic variation in natural populations of Orobanche foetida from Spain and Tunisia

RAPD markers were used to study variation among Orobanche foetida and Orobanche foetida ssp. lusitanicus populations growing either on wild or cultivated hosts. O. foetida populations from agricultural environments were collected on chickpea (Cicer arietinum L.) and faba bean (Vicia faba L.) fields in Tunisia. O. foetida and O. foetida ssp. lusitanicus populations on wild hosts were sampled in Spain. Jaccard's coefficient of similarity was calculated conisdering 126 RAPD amplification products and populations were grouped by cluster analysis using the UPGMA method. Although the AMOVA analysis revealed a substantial internal variation among individuals within Tunisian O. foetida populations (75.4 %), there was a significant divergence btween the two hosts considered (24.6 %). The dendrogram also shows a clear differentiation between O. foetida populations infecting different hosts (C. arietinum and V. faba) and among populations infecting crops and wild flora.

Orobanche foetida; RAPD; AMOVA; genetic diversity

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