Epidemiology of carbapenem-hydrolyzing oxacillinases, metallo-beta-lactamases and common insertion sequences in Acinetobacter baumannii from Bosnia and Herzegovina (CROSBI ID 658657)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Ibrahimagić, Amir ; Petrović, Tanja ; Jakovac, Sanja ; Slačanac, Domagoj ; Uzunović, Selma ; Bedenić, Branka ; Luxner, Josefa
engleski
Epidemiology of carbapenem-hydrolyzing oxacillinases, metallo-beta-lactamases and common insertion sequences in Acinetobacter baumannii from Bosnia and Herzegovina
Multidrug-resistant Acinetobacter baumannii (MDR-AB) is an important threat for critically ill patients. It can infect the respiratory tract, blood, soft tissues, urinary tract and central nervous system. Recently, carbapenem- resistance was observed in A. baumannii clinical isolates from Bosnia and Herzegovina. This prompted us to analyze these isolates with regards to genotypic diversity, antibiotic susceptibility and occurrence of acquired carbapenem resistance genes. Twelve carbapenem- resistant isolates were collected at a University hospital during two different periods of 2011 and 2015-2016: four isolates in 2011 and eight isolates 2015-2016 and compared to determine the dynamic changes in carbapenemase resistance mechanisms and population structure. All twelve isolates were positive for intrinsic blaOXA-51-like, nine for blaOXA-40-like and one for the blaOXA-23-like gene. ISAba1 was found upstream of blaOXA-51 in all and upstream of blaOXA-23-like gene in one isolate. Sequencing of the selected PCR products revealed the presence of OXA-72 β- lactamase (strain 1) and OXA-23 β-lactamase (strain 41). WGS of the selected isolate (AB 5) revealed the presence of blaOXA-72, chromosomal genes blaOXA-69 and blaADC. Moreover, the aac (3)-1a and aadA1 genes encoding aminoglycoside resistance, and sul1encoding sulphonamide resistance were identified. PFGE and rep-PCR revealed two clones containing highly similar isolates positive for OXA-40-like ; one from 2011 and the other from 2015-2016. Implementation of hospital hygiene measures, screening of the patients on admission for carriage of MDR-AB, and the early and accurate detection, with restriction of antibiotic use should be recommended to control the spread of these important hospital pathogenes. To our knowledge, this is the first report of A. baumannii isolates producing carbapenem- hydrolyzing oxacillinases (CHDL) from Bosnia and Herzegovina.
Acinetobacter baumannii, carbapenems, resistance, oxacillinases
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Podaci o prilogu
P0391-119.
2017.
objavljeno
Podaci o matičnoj publikaciji
Podaci o skupu
27th European congress of clinical microbiology and infectious diseases
poster
22.04.2017-25.04.2017
Beč, Austrija