engleski

Comparison of serological procedures for diagnosis of infection with Chlamydophila sp. in bovines

In order to diagnose chlamydiosis caused by bacteria Chlamydophila sp., the humoral immunity response in domestic bovines was examined. A possible difference in titre of specific antibodies between latently, acutely and chronically infected animals was also researched. At the same time we confirmed the applicability and reliability of complement-fixation tests (CFT) and enzyme– linked immunosorbent assay (ELISA) on the basis of their specificity, sensitivity and comparativeness. Out of 276 bovines examined, originated from 10 herds on the Croatian territory, CFT revealed Chlamydophila sp. specific antibodies 14.2% of sera samples, ELISA in 35.8%, and indirect immunofluorescent test (IIF) in 27.1% sera samples. These results suggest that CFT, ELISA and IIF can be use as reliable serological methods for diagnostics of chlamydiosis and they are in accordance with Office International des Epizooties (OIE) recommendations.

Chlamydophila; diagnostics; serology; bovine; infection; Croatia

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