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izvor podataka: crosbi

Bioprospecting for Genes Encoding Hydrocarbon-Degrading Enzymes from Metagenomic Samples Isolated from Northern Adriatic Sea Sediments (CROSBI ID 248852)

Prilog u časopisu | kratko priopćenje | međunarodna recenzija

Gacesa, Ranko ; Baranasic, Damir ; Starcevic, Antonio ; Diminic, Janko ; Korlević, Marino ; Najdek-Dragić, Mirjana ; Blažina, Maria ; Oršolić, Davor ; Kolesarić, Domagoj ; Long, Paul F. et al. Bioprospecting for Genes Encoding Hydrocarbon-Degrading Enzymes from Metagenomic Samples Isolated from Northern Adriatic Sea Sediments // Food technology and biotechnology, 56 (2018), 2; 270-277. doi: 10.17113/ftb.56.02.18.5393

Podaci o odgovornosti

Gacesa, Ranko ; Baranasic, Damir ; Starcevic, Antonio ; Diminic, Janko ; Korlević, Marino ; Najdek-Dragić, Mirjana ; Blažina, Maria ; Oršolić, Davor ; Kolesarić, Domagoj ; Long, Paul F. ; Cullum, John ; Hranueli, Daslav ; Orlić, Sandi ; Zucko, Jurica ;

engleski

Bioprospecting for Genes Encoding Hydrocarbon-Degrading Enzymes from Metagenomic Samples Isolated from Northern Adriatic Sea Sediments

Three metagenomic libraries were constructed using surface sediment samples from the northern Adriatic Sea. Two of the samples were taken from a highly polluted and an unpolluted site respectively. The third sample from a polluted site had been enriched using crude oil. The results of the metagenome analyses were incorporated in the REDPET relational database (http://redpet.bioinfo.pbf.hr/REDPET), which was generated using the previously developed MEGGASENSE platform. The database includes taxonomic data to allow the assessment of the biodiversity of metagenomic libraries and a general functional analysis of genes using hidden Markov model (HMM) profiles based on the KEGG database. A set of 22 specialised HMM-profiles was developed to detect putative genes for hydrocarbon-degrading enzymes. Use of these profiles showed that the metagenomic library generated after selection on crude oil had enriched genes for aerobic n-alkane degradation. The use of this system for bioprospecting was exemplified using potential alkB and almA genes from this library.

oil pollution ; n-alkane degradation, database

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Podaci o izdanju

56 (2)

2018.

270-277

objavljeno

1330-9862

1334-2606

10.17113/ftb.56.02.18.5393

Povezanost rada

Biologija, Biotehnologija

Poveznice
Indeksiranost