engleski

CHLAMYDOPHILOSIS (CHLAMYDOPHILA PSITTACI) IN CANARIES AND FINCHES - BIOLOGICAL EXPERIMENT

In Slovenia, immune reactivity for Chlamydophila psittaci was monitored in canaries (Serinus domesticus) and finches (Poephila guttata and Lonchura domestica) for the period 1991 to 2000. Indirect immunofluorescence was used to test over 1000 specimens of birds tested. Specific IgG antibodies were found in 0.5% of canaries. Experimentally induced infection with C. psittaci in canaries and finches enabled study of the clinical, pathoanatomical and pathohistological pictures of chlamydophilosis in these animal species. C. psittaci strains used for experiment were first isolated from cockatiel (Nymphicus hollandicus) and propagated in BALB/c laboratory mice strain and in McCoy cell cultures. DNA was also extracted and RFLP-PCR was done. Infection of canaries and finches was proven with direct immunofluorescence and encime-immunoassay of organ smears and imprints and with serological methods, indirect immunofluorescence and complement fixation. Futhermore, the presented results include serological results for breeders of canaries and finches exposed to infection from C. psittaci. The results of tests for the presence of specific antibodies obtained using the microimmunofluorescence method and results for the correlation between this infection in people and birds. Among the breeders of canaries and finches we founf 4.6% immunologically reactice subjects. Testing of two successive samples was used to demonstrate an increase in IgG and IgA antibodies. However, IgM characteristic of acute infection were never confirmed.

chlamydophilosis; Chlamydiphila psittaci; canaries; finches; McCoy cell culture; laboratory mice; presence of antibody; immunofluorescence; encime-immunoassay; pathological research

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