engleski

Effects of different n6/n3 ratios and supplementation with DHA and EPA on the testicular histology and lipogenesis in streptozotocin treated rats

The aim of this study was to investigate the possible influence of different dietary n6/n3 ratios and DHA/EPA addition on the testis histology, antioxidative status and lipogenesis of streptozotocin treated rats. Diabetes was induced by a single intraperitoneal injection of streptozotocin (55 mg/kg). The rats were divided into 5 groups: CON (n6/n3 ratio, 7), CON-DM1 (STZ ; n6/n3 ratio, 7), N3-DM1 (STZ ; n6/n3 ratio, 1), N6-DM1 (STZ ; n6/n3 ratio, 60) and DHA-DM1 (STZ ; n6/n3 ratio, 1 ; added DHA/EPA). Antioxidative status was improved in the DHA-DM1 group. Seminiferous tubule diameter, testicular pathohistological scoring and total lipid content were improved in the N6-DM1 group compared to the other streptozotocin treated groups. Streptozotocin treatment induced changes in testis fatty acid profile depending on n6/n3 ratio. The fatty acid profile of N6-DM1 group was characterized by similar or increased values for n6 fatty acids compared to the CON group, while the DHA- DM1 group had the lowest content of n6 fatty acids. The content of n3 fatty acids was increased in the N3-DM1 and DHA-DM1 groups. These results suggest that a n6/n3 ratio could significantly influence testicular antioxidative status, histology and lipogenesis, and that these effects vary depending on the supplemented fatty acid.

lipid peroxidation, n6/n3 ratio, streptozotocin, testicular lipogenesis

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