Injectable chitosan-hydroxyapatite hydrogels promote the osteogenic differentiation of mesenchymal stem cells (CROSBI ID 252251)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Ressler, Antonia ; Ródenas-Rochina, Joaquin ; Ivanković, Marica ; Ivanković, Hrvoje ; Rogina, Anamarija ; Gallego Ferrer, Gloria
engleski
Injectable chitosan-hydroxyapatite hydrogels promote the osteogenic differentiation of mesenchymal stem cells
Injectable hydrogels have emerged as promising biomaterials for tissue engineering applications. The goal of this study was to evaluate the potential of a pH-responsive chitosan- hydroxyapatite hydrogel to be used as a threedimensional support for encapsulated mesenchymal stem cells (MSCs) osteogenic differentiation. In vitro enzymatic degradation of the hydrogel, during 28 days of incubation, in simulated physiological condiditons, was characterized by swelling measurements, molecular weight determination and SEM analysis of hydrogel microstructure. Osteogenic differentiation of encapsulated MSCs was confirmed by osteogenic Runx2, collagen type I and osteocalcin immunostaining and alkaline phosphatase quantification. The deposition of late osteogenic markers (calcium phosphates) detected by Alizarin red and von Kossa staining indicated an extracellular matrix mineralization.
chitosan ; differentiation ; hydrogel ; hydroxyapatite ; MSCs ; osteogenesis
Glavni autori: Ressler Antonia i Ivanković Marica
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Podaci o izdanju
197
2018.
469-477
objavljeno
0144-8617
1879-1344
10.1016/j.carbpol.2018.06.029
Povezanost rada
Biologija, Kemijsko inženjerstvo, Temeljne tehničke znanosti