engleski

Assessment of population diversity in wild Rubus idaeus L. populations from Serbia

Genus Rubus L. (Rosaceae), with more than 700 species, is one of the largest genera in plant kingdom. Raspberry (Rubus idaeus L.) is diploid and belongs to subgenus Idaeobatus. Raspberry leaves are used in traditional medicine for treatment of various disorders most commonly related to menstruation, parturition, and ailments of the gastrointestinal tract and other purposes, while fruit is used as food. In this work we used AFLP markers to analyse intrapopulation diversity and interpopulation relationships of seven wild growing populations of R. idaeus from Serbia, that can be grouped into three spatial groups as well as into two altitude groups. Four AFLP combinations yield a total of 247 polymorphic bands in 133 R. idaeus specimens. Data analysis revealed similar levels of diversity across populations. Population from Mt. Ozren had both the highest diversity and the highest frequency down-weighted marker values. AMOVA analyses further showed high intrapopulation diversity, while population differentiation was low. The partition among spatial groups was not significant while the partition between altitude groups was highly significant. BAPS grouped the samples into three clusters of which to cluster C belong exclusively individuals originating from population Ozren. Clusters were almost identical with or without spatial coordinates. In analysed populations altitude had a stronger influence on differentiation than horizontal distance and that can be attributed to different phenology at different altitudes. Our results indicate that AFLP markers are reliable technique for assessing genetic diversity and relations between R. idaeus populations.

raspberry, AFLP, population variability

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