engleski

The human urinary microbiome in bladder cancer

Backgrounds Although traditionally considered sterile, recent studies have shown that bladder, similar to other body sites, harbours a d iverse microbial population which could be a contributing factor to urinary bladder pathogenesis. Most of the studies published so far were analysing females, proposing an association of female urinary microbiota with disorders such as urgency urinary inco ntinence and urinary tract infection. Knowledge of male urinary microbiota is very limited, and the link between urinary microbiota and bladder cancer, known to be three times more common in men then in women, has not been explored yet. Objectives The main goal of our study was to examine male urinary microbiome in bladder cancer patients and compare it with urinary microbiome of healthy controls. Methods Urine samples were obtained from male bladder cancer patients and healthy controls. DNA was isolated f rom urines and enriched for bacterial 16S V4 rDNA region by PCR amplification. The amplicons were sequenced on Illumina MiSeq platform and data analysis was performed in collaboration with Second Genome’s Microbial Profiling Service. Conclusions The urine microbiome from bladder cancer patients and healthy controls did not exhibit significant alternations at a microbial community level. Operational taxonomic units that differed significantly between the two groups included the genera Kocuria , Actinobaculum , Fusobacterium , Micrococcus , Pyramidobacter and Corynebacterium which were more abundant in bladder cancer, and the genera Streptococcus and Veillonella , enriched in healthy samples. Further studies with larger cohort are needed to establish the role of ur inary microbiota in bladder cancer development and treatment response.

microbiom, bladder cancer

nije evidentirano